Mucosal Inflammation Group

Overview

Our research group resides within the Mucosal Immunology Laboratory of the Pediatrics Department. Granulocytes migrate across mucosal barriers during infectious and idiopathic disease. This process can become overly aggressive, lacking appropriate resolution and culminating in tissue damage. Our group seeks to better understand the molecular mechanisms that underlie granulocyte breach of the mucosal barrier with the objective of therapeutically controlling this process in the context of mucosal inflammatory disease. In addition, we are actively investigating the diverse array of threats, including microbes, toxins, and allergens that can impact the mucosal surface by damaging, compromising barrier integrity, and/or eliciting host inflammatory responses.

Publications

Original Articles

1. Fischer, C.P., Bode, B.P., Hurley, B.P. Souba W.W. Alterations in oxidative metabolism and glutamine transport support glucose production in the tumor-influenced hepatocyte. J Surg Res. 1997; 69(2):379-84. PMID: 9224411
2. Hurley, B.P., Jacewicz, M., Thorpe, C.M., Lincicome, L.L., King, A.J., Keusch, G.T., Acheson, D.W.K. Shiga toxins 1 and 2 translocate differently across polarized intestinal epithelial cells. Infect Immun. 1999; 67(12):6670-7. PMID: 10569789
3. Thorpe, C.M., Hurley, B.P., Lincicome, L.L., Jacewicz, M.S., Keusch, G.T., Acheson D.W.K.. Shiga toxins stimulate secretion of interleukin-8 from intestinal epithelial cells. Infect Immun. 1999; 67(11):5985-93. PMID: 10531258
4. Thorpe, C.M., Flaumenhaft, R., Hurley, B., Jacewicz, M., Acheson, D.W.K., Keusch, G.T. Shiga toxins do not directly stimulate alpha-granule secretion or enhance aggregation of human platelets. Acta Haematol. 1999; 102(1):51-5. PMID: 10473889
5. Hurley, B.P., Thorpe, C.M., Acheson, D.W.K. Shiga toxin translocation across intestinal epithelial cells is enhanced by neutrophil transmigration. Infect. Immun. 2001; 69(10):6148-55. PMID: 11553554
6. Thorpe, C.M., Smith, W.E., Hurley, B.P., Acheson D.W.K. Shiga toxins induce, superinduce, and stabilize a variety of C-X-C chemokine mRNA in intestinal epithelial cells resulting in increased chemokine expression. Infect. Immun. 2001; 69(10):6140-7. PMID: 11553553
7. Bode, B.P., Fuchs, B.C., Hurley, B.P., Conroy J.L., Suetterlin, J.E., Tanabe, K.K., Rhoads D.B., Abcouwer, S.F., Souba, W.W.. Molecular and functional analysis of glutamine uptake in human hepatoma and liver-derived cells. Am J Physiol Gastrointest Liver Physiol. 2002; 283(5):G1062-73. PMID: 12381519
8. Mrsny, R.J., Gewirtz, A.T. , Siccardi,,D., Savidge, T.C., Hurley, B.P., Madara, JL, McCormick, B.A.. Identification of hepoxilin A3 in inflammatory events: A required role in neutrophil migration across the intestinal epithelia. Proc. Natl. Acad. Sci. USA 2004; 101: 7421-7426. PMID: 15123795
9. Spacek L.A., Hurley B.P., Acheson D.W., Granok A., Currie A., Doing K., Sears C.L.. Shiga Toxin-Producing Escherichia coli as a Possible Etiological Agent of Chronic Diarrhea. Clin Infect Dis. 2004; 39(5):E46-8. PMID: 15356801
10. 10. Hurley, B.P., Siccardi, D., Mrsny, R.J., McComick, B.A.. PMN transepithelial migration induced by Pseudomonas aeruginosa requires the eicosinoid hepoxilin A3. J Immunol. 2004; 173(9):5712-20. PMID: 15494523
11. Hurley, B.P., Williams, N.L., McCormick, B.A.. Involvement of Phospholipase A2 in Pseudomonas aeruginosa Mediated PMN Trans-epithelial Migration. Am J Physiol Lung Cell Mol Physiol. 2006; 290(4):L703-9. PMID: 16272174
12. Köhler, H., Sakaguchi1, T., Hurley, B.P., Kase, B.A., Reinecker, H.C., McCormick, B.A. Salmonella regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Am J Physiol Gastro. and Liver Physiol. 2007; 293(1):G178-87. PMID: 17615177
13. Hurley, B.P., Sin, A., McCormick, B.A. Adhesion Molecules Involved in Hepoxilin A3 Mediated PMN Trans-epithelial Migration. Clin. and Exp. Immuno. 2008; 151(2):297-305. PMID: 18005361
14. Mumy, K.L., Bien, J.D., Pazos, M.A., Gronert, K., Hurley, B.P., McCormick, B.A. Distinct Isoforms of Phospholipase A2 mediate the ability of Salmonella enterica serotype Typhimurium and Shigella flexneri to induce the transepithelial migration of neutrophils. Infect. Immun. 2008; 76(8):3614-27. PMID: 18505810
15. 15. Hurley, B.P., Goodman, A.L., Murphy, P., Lory, S., McCormick, B.A.. The Two-Component Sensor Response Regulator RoxR / RoxS Plays a Role in Pseudomonas aeruginosa Interactions with Airway Epithelial Cells. Microbes & Infection. 2010; 12(3):190-8. PMID: 19961952
16. Hurley, B.P., Pirzai, W., Mumy, K.L., Gronert, K., McCormick B.A.. Selective Eicosanoid Generating Capacity of Cytoplasmic Phospholipase A2 in Pseudomonas aeruginosa Infected Epithelial Cells. Am J Physiol Lung Cell Mol Physiol. 2011 300(2):L286-94. PMID: 21097525

Reviews and Chapters

1. Thorpe CM, Hurley BP, Acheson DWK. Shiga Toxin Interactions with the Intestinal Epithelium. In, Philpott, D.J., ed. Methods in Molecular Medicine. Totowa, NJ: Humana Press, 2002: 273:263-273. PMID: 12375435
2. Hurley, BP and BA McCormick (2003) Translating tissue culture results into animal models: the case of Salmonella typhimurium. Trends in Microbiol. 11(12);562-9. PMID: 14659688
3. Hurley, BP and BA McCormick (2004) Intestinal Epithelial Defense Systems Protect Against Bacterial Threats. Current Gastroenterology Reports. 6(5):355-61. PMID: 15341710
4. Hurley, BP and BA McCormick (2008) Multiple Roles of Phospholipase A2 during Lung Infection and Inflammation. Infect. Immun. 76(6):2259-2272 PMID: 18411286
   
   (Compiled August, 2011)